The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_213963.2 | NP_999128.2 | 12 - 12 | 2290 | 96 |
| XM_021100438.1 | XP_020956097.1 | - | 3062 | 96 | |
| XM_021100439.1 | XP_020956098.1 | - | 2787 | 96 | |
| XM_021100440.1 | XP_020956099.1 | - | 5078 | 96 | |
| XM_021100441.1 | XP_020956100.1 | - | 3183 | 96 | |
| XM_021100442.1 | XP_020956101.1 | - | 5553 | 96 | |
| XM_021100444.1 | XP_020956103.1 | - | 4549 | 96 | |
| GenBank mRNA | AB106108.1 | - | 12 - 12 | 2290 | 96 |
| AH013165.2 | - | 12 - 12 | 2348 | 96 | |
| AK235681.1 | - | 12 - 12 | 2427 | 96 | |
| AK393283.1 | - | 13 - 13 | 2479 | 96 | |
| AK393759.1 | - | 12 - 12 | 2378 | 96 | |
| AK398499.1 | - | 5 - 5 | 775 | 96 | |