Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: PIFI
Entrez Gene ID: 820827
Gene Name: post-illumination chlorophyll fluorescence increase
Gene Aliases: AT3G15840, post-illumination chlorophyll fluorescence increase
Chromosome Location: Chr.3: 5356587 - 5358596 on Build TAIR10.1

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001035629.1	NP_001030706.1	4 - 5	362	80
	NM_001035630.1	NP_001030707.1	4 - 5	429	80
	NM_001125170.1	NP_001118642.1	3 - 4	450	80
	NM_001202975.1	NP_001189904.1	4 - 5	539	80
	NM_112454.3	NP_566528.1	4 - 5	526	80
GenBank mRNA	AY035025.1	-	4 - 5	385	80
GenBank mRNA	AY133879.1	-	4 - 5	348	80