Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: HIT1
Entrez Gene ID: 841472
Gene Name: Membrane trafficking VPS53 family protein
Gene Aliases: ARABIDOPSIS THALIANA VPS53 HOMOLOG, AT1G50500, ATVPS53, HEAT-INTOLERANT 1, VPS53
Chromosome Location: Chr.1: 18707924 - 18716118 on Build TAIR10.1

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001198259.1	NP_001185188.1	20 - 21	2073	113
	NM_001333440.1	NP_001322424.1	19 - 20	2303	113
	NM_001333441.1	NP_001322422.1	20 - 21	2090	113
	NM_001333442.1	NP_001322423.1	20 - 21	2058	113
	NM_103933.6	NP_564573.3	20 - 21	2199	113
GenBank mRNA	AK228745.1	-	20 - 21	2054	113