Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: mfr
Entrez Gene ID: 39016
Gene Name: misfire
Gene Aliases: CG5747, Dmel\CG5747, Dmel_CG5747, Otoferlin
Chromosome Location: Chr.3L: 8905918 - 8912671 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0266757

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001144443.2	NP_001137915.1	8 - 9	4039	134
	NM_001144444.2	NP_001137916.1	8 - 9	4528	134
	NM_001144445.2	NP_001137917.1	7 - 8	3998	134
	NM_001144446.2	NP_001137918.1	7 - 8	4527	134
	NM_001144447.2	NP_001137919.1	8 - 9	4065	134
	NM_001144448.2	NP_001137920.1	7 - 8	4111	134
	NM_001144449.2	NP_001137921.1	7 - 8	3975	134