The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_057855.6 | NP_477203.1 | 3 - 3 | 121 | 140 |
NM_166437.4 | NP_726053.1 | 2 - 2 | 181 | 140 | |
NM_166438.4 | NP_726054.1 | 2 - 2 | 96 | 140 | |
GenBank mRNA | KX531539.1 | - | 1 - 1 | 68 | 140 |
U40226.1 | - | 2 - 2 | 97 | 140 |