The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_005384.2 | NP_005375.2 | 2 - 2 | 246 | 80 |
| GenBank mRNA | AK313970.1 | - | 2 - 2 | 62 | 80 |
| AW163045.1 | - | 2 - 2 | 181 | 80 | |
| BQ937243.1 | - | 2 - 2 | 246 | 80 | |
| S79880.1 | - | 2 - 2 | 64 | 80 | |
| U26173.1 | - | 2 - 2 | 64 | 80 | |
| X64318.1 | - | 2 - 2 | 64 | 80 | |