The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_002787.4 | NP_002778.1 | 5 - 5 | 477 | 123 |
| GenBank mRNA | AK222976.1 | - | 5 - 5 | 445 | 123 |
| AK290654.1 | - | 5 - 5 | 452 | 123 | |
| AU120930.1 | - | 5 - 5 | 452 | 123 | |
| BC002900.2 | - | 4 - 4 | 403 | 123 | |
| BC047697.1 | - | 5 - 5 | 431 | 123 | |
| BX641097.1 | - | 4 - 4 | 368 | 123 | |
| CB990982.1 | - | 5 - 5 | 520 | 123 | |
| CR450317.1 | - | 5 - 5 | 429 | 123 | |
| D00760.1 | - | 5 - 5 | 429 | 123 | |