The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_005362.3 | NP_005353.1 | 1 - 1 | 24 | 97 |
| XM_005274676.3 | XP_005274733.1 | 1 - 1 | 34 | 161 | |
| GenBank mRNA | BC005963.1 | - | 1 - 1 | 24 | 97 |