The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_001244713.1 | NP_001231642.1 | 4 - 4 | 568 | 75 |
NM_001244724.1 | NP_001231653.1 | 4 - 4 | 558 | 75 | |
NM_002874.4 | NP_002865.1 | 4 - 4 | 883 | 75 | |
GenBank mRNA | AK125226.1 | - | 5 - 5 | 787 | 75 |
AK223479.1 | - | 4 - 4 | 835 | 75 | |
AK293532.1 | - | 4 - 4 | 875 | 75 | |
AK297986.1 | - | 4 - 4 | 528 | 75 | |
AK316189.1 | - | 4 - 4 | 483 | 75 | |
AL540969.3 | - | 4 - 4 | 854 | 75 | |
AY313777.1 | - | 4 - 4 | 558 | 75 | |
BC020973.2 | - | 4 - 4 | 539 | 75 | |
BI460482.1 | - | 4 - 4 | 575 | 75 | |
D21090.1 | - | 4 - 4 | 802 | 75 |