Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Sema3b
Entrez Gene ID: 20347
Gene Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Gene Aliases: SemA, Semaa, sema5, semaV
Chromosome Location: Chr.9: 107597674 - 107609303 on Build GRCm38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001042779.2	NP_001036244.1	17 - 18	2050	68
	NM_001291537.1	NP_001278466.1	17 - 18	2314	68
	NM_001291538.1	NP_001278467.1	17 - 18	2308	68
	NM_001291539.1	NP_001278468.1	16 - 17	1991	68
	NM_009153.3	NP_033179.2	15 - 16	1860	68
	NR_111986.1		15 - 16	1803	68
GenBank mRNA	AK014333.1	-	15 - 16	1739	68
	AK033597.1	-	16 - 17	2001	68
	AK037034.1	-	17 - 18	2039	68
	AK043634.1	-	16 - 17	1944	68
	AK048364.1	-	16 - 17	2272	68
	AK148232.1	-	17 - 18	2011	68
	AK205569.1	-	1 - 2	91	68
	BB116052.2	-	4 - 5	315	68
	BC090669.1	-	16 - 17	1971	68
	BC150758.1	-	14 - 15	1706	68
	X85990.1	-	15 - 16	1857	68