The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_019685.2 | NP_062659.1 | 9 - 9 | 1178 | 142 |
| GenBank mRNA | AF100694.1 | - | 9 - 9 | 1217 | 142 |
| AK012170.1 | - | 9 - 9 | 1123 | 142 | |
| AK132783.1 | - | 9 - 9 | 1160 | 142 | |
| AK146258.1 | - | 9 - 9 | 1171 | 142 | |
| AK146374.1 | - | 9 - 9 | 1171 | 142 | |
| AK156078.1 | - | 9 - 9 | 1148 | 142 | |
| BC004718.1 | - | 9 - 9 | 1144 | 142 | |