The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_029376.2 | NP_083652.2 | 5 - 5 | 730 | 147 |
GenBank mRNA | AK006439.1 | - | 5 - 5 | 734 | 147 |