The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_053345.1 | NP_445797.1 | 3 - 3 | 288 | 91 |
| XM_006243380.2 | XP_006243442.1 | 4 - 4 | 438 | 91 | |
| GenBank mRNA | AF000944.1 | - | 3 - 3 | 288 | 91 |
| BC168727.1 | - | 5 - 5 | 472 | 91 | |
| FQ209546.1 | - | - | 443 | 91 | |
| FQ212213.1 | - | 4 - 4 | 423 | 91 | |
| FQ221947.1 | - | 4 - 4 | 429 | 91 | |