The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001244209.1 | NP_001231138.1 | 4 - 4 | 426 | 77 |
| GenBank mRNA | AF034988.1 | - | 2 - 2 | 150 | 77 |
| AK234945.1 | - | 4 - 4 | 417 | 77 | |
| AK391956.1 | - | 4 - 4 | 427 | 77 | |
| AK392323.1 | - | 4 - 4 | 364 | 77 | |
| AK400416.1 | - | 4 - 4 | 408 | 77 | |
| FS666815.1 | - | 4 - 4 | 427 | 77 | |