The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001044618.1 | NP_001038083.1 | 9 - 9 | 1048 | 98 |
| XM_005668230.2 | XP_005668287.1 | - | 1401 | 98 | |
| XM_005668231.3 | XP_005668288.1 | - | 1460 | 98 | |
| XM_005668232.3 | XP_005668289.1 | - | 1380 | 98 | |
| XM_005668236.3 | XP_005668293.1 | - | 1061 | 98 | |
| XM_013980354.2 | XP_013835808.1 | - | 1247 | 98 | |
| GenBank mRNA | AB527065.1 | - | 8 - 8 | 903 | 98 |
| AK233202.1 | - | 10 - 10 | 1197 | 98 | |
| AK393235.1 | - | 10 - 10 | 1278 | 98 | |
| AK393546.1 | - | 8 - 8 | 901 | 98 | |
| AK400494.1 | - | 11 - 11 | 1364 | 98 | |
| DQ474067.1 | - | 9 - 9 | 1048 | 98 | |