Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: TOP1ALPHA
Entrez Gene ID: 835623
Gene Name: DNA topoisomerase I alpha
Gene Aliases: AT5G55300, DNA topoisomerase 1 beta, DNA topoisomerase I alpha, MGO1, MGOUN 1, MTE17.1, MTE17_1, TOP1, TOP1BETA, TOPOISOMERASE 1
Chromosome Location: Chr.5: 22424502 - 22429514 on Build TAIR10.1

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001203614.1	NP_001190543.1	13 - 14	2547	115
	NM_001203615.1	NP_001190544.1	7 - 8	1484	115
	NM_124912.5	NP_200341.1	13 - 14	2559	115
GenBank mRNA	X57544.1	-	-	2484	115