The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | XM_005637529.3 | XP_005637586.1 | 2 - 2 | 813 | 190 |
XM_534958.6 | XP_534958.2 | 2 - 2 | 810 | 190 | |
GenBank mRNA | AJ628248.1 | - | 2 - 2 | 575 | 190 |
AJ628249.1 | - | 2 - 2 | 575 | 190 |