The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001128186.1 | NP_001121658.1 | - | 1003 | 136 |
| XM_022426499.1 | XP_022282207.1 | - | 1181 | 136 | |
| XM_022426500.1 | XP_022282208.1 | - | 1102 | 136 | |
| XM_022426501.1 | XP_022282209.1 | - | 1065 | 136 | |
| XM_022426502.1 | XP_022282210.1 | - | 976 | 136 | |
| GenBank mRNA | EU541502.1 | - | - | 1003 | 136 |