Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Galphaq
Entrez Gene ID: 36384
Gene Name: G protein alpha q subunit
Gene Aliases: CG17759, DGalpha[[q]], DGalphaq, DGq, DGqalpha, Dgq, DmGalpha[[q]], Dmel\CG17759, Dmel_CG17759, Dromel_CG17759_galpha49b_mORF, G alpha 49B, G?q, GNAQ/11/14, G[[a]]q, G[[alphaq]], G[[q]], G[[q]]alpha, G[[qalpha]], G[q], Galpha, Galpha49B, Galpha49b, Galpha[[q]], Gaq, Gq, Gqa-3, Gqalpha, Gqalpha[[e]], Gqalphae, Gqalpha{e}, dG[[alphaq]], dG[[q]]alpha, dGaalpha, dGq, dGqa-49b, dGqalpha, dGqalpha-3, dGqalpha-49b, dgq, dgqalpha, galpha49B, sast
Chromosome Location: Chr.2R: 12612740 - 12623197 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0004435

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_165919.2	NP_725191.1	5 - 6	1763	75
	NM_165920.3	NP_725192.2	4 - 5	951	75
	NM_165921.2	NP_725193.1	4 - 5	951	75
	NM_165922.2	NP_725194.1	5 - 6	800	75
	NM_165923.2	NP_725195.1	5 - 6	1730	75
	NM_165925.3	NP_725197.2	4 - 5	984	75
GenBank mRNA	AY118511.1	-	5 - 6	1731	75
GenBank mRNA	U31092.1	-	5 - 6	1758	75

Gene Symbol: CG45086
Entrez Gene ID: 19835902
Gene Name: CG45086-PA
Gene Aliases: Dmel\CG45086, Dmel_CG45086, Dromel_CG17759_galpha49b_uORF
Chromosome Location: Chr.2R: 12612740 - 12619820 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0266487

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001299427.1	NP_001286356.1	5 - 6	1730	75
RefSeq	NM_001299428.1	NP_001286357.1	5 - 6	1763	75