Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: eIF4A
Entrez Gene ID: 33835
Gene Name: eukaryotic translation initiation factor 4A
Gene Aliases: 2439, 9075, CG9075, Dm-eIF4A, DmRH2, Dmel\CG9075, Dmel_CG9075, EIF-4A, EIF-4a, EIF4A, EiF4A, Eif-4a, Eif4a, L(2L)162, Su(dpp)YE9, anon-26Aa, anon26A, deIF4A, eIF 4A, eIF-4A, eIF-4a, eIF-4a-I, eIF4A-I, eIF4AI, eIF4a, eiF4A, eif-4a, eif4A, eif4a, l(2)02439, l(2)162, l(2)26Ac, l(2)gdh-4, l(2)k01501, l(2L)162, pecan
Chromosome Location: Chr.2L: 5981764 - 5985909 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0001942

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001258978.3	NP_001245907.1	4 - 5	1141	93
	NM_001273188.1	NP_001260117.1	4 - 5	1297	93
	NM_057247.5	NP_476595.1	4 - 5	1141	93
	NM_164668.3	NP_723137.1	4 - 5	1141	93
	NM_164669.3	NP_723138.1	4 - 5	1297	93
	NM_164670.4	NP_723139.1	4 - 5	1528	93
GenBank mRNA	AF145621.1	-	-	4244	93
	AY069283.1	-	4 - 5	1136	93
	AY121623.1	-	-	2032	93
	BT099924.1	-	4 - 5	1264	93