Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Nhe3
Entrez Gene ID: 33939
Gene Name: Na[+]/H[+] hydrogen exchanger 3
Gene Aliases: CG11328, DmNHE3, Dmel\CG11328, Dmel_CG11328, NHE3, nhe3
Chromosome Location: Chr.2L: 6677835 - 6684364 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0028703

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001103635.3	NP_001097105.3	2 - 3	766	59
	NM_001144329.2	NP_001137801.1	2 - 3	759	59
	NM_001273217.1	NP_001260146.1	2 - 3	259	59
	NM_001273218.1	NP_001260147.1	2 - 3	759	59
	NM_135220.3	NP_609064.3	2 - 3	759	59
	NM_164701.2	NP_723202.2	2 - 3	759	59
GenBank mRNA	BT124889.1	-	2 - 3	259	59