Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: step
Entrez Gene ID: 35425
Gene Name: steppke
Gene Aliases: CG11628, CG11633, CYH1, Dmel\CG11628, Dmel_CG11628, GPH, GRP1, GRP1/cytohesin 1, Grp1, Step, cytohesin/GRP1, l(2)SH0323, l(2)SH2 0323, l(2)k08110, stepk
Chromosome Location: Chr.2L: 21740041 - 21757466 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0086779

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001042910.3	NP_001036375.2	6 - 7	1758	73
	NM_001169570.2	NP_001163041.1	5 - 6	2019	73
	NM_001273749.1	NP_001260678.1	7 - 8	2487	73
	NM_136276.4	NP_610120.1	5 - 6	710	73
GenBank mRNA	AY071322.1	-	5 - 6	644	73
	BT072836.1	-	5 - 6	662	73
	BT124891.1	-	7 - 8	2386	73