Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Idh
Entrez Gene ID: 44291
Gene Name: Isocitrate dehydrogenase
Gene Aliases: CG7176, CT22171, Dmel\CG7176, Dmel_CG7176, ICDH, IDH, IDH (CG7176), IDH-NADP, Idh-NADP, cIdh, idh, isocitrate dehydrogenase, l(3)L3852
Chromosome Location: Chr.3L: 8356404 - 8360906 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0001248

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001144438.2	NP_001137910.2	3 - 3	1872	109
	NM_001144439.2	NP_001137911.1	3 - 3	1797	109
	NM_143787.3	NP_652044.1	3 - 3	1842	109
	NM_168265.2	NP_729366.1	2 - 2	1924	109
	NM_168266.2	NP_729367.1	2 - 2	1540	109
	NM_168267.2	NP_729368.1	2 - 2	1514	109
	NM_168268.3	NP_729369.2	3 - 3	1674	109
	NM_168269.2	NP_729370.1	2 - 2	1544	109
	NM_176298.2	NP_788476.1	3 - 3	1707	109
GenBank mRNA	AY047505.1	-	2 - 2	1931	109
GenBank mRNA	BT099506.1	-	2 - 2	1517	109