Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Zasp66
Entrez Gene ID: 38988
Gene Name: Z band alternatively spliced PDZ-motif protein 66
Gene Aliases: CG6416, Dmel\CG6416, Dmel_CG6416, ZASP66, Zaps66, anon-WO0153538.30
Chromosome Location: Chr.3L: 8624371 - 8638813 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0035917

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001169911.1	NP_001163382.1	4 - 5	958	74
	NM_001169913.1	NP_001163384.1	4 - 5	958	74
	NM_001169914.3	NP_001163385.1	4 - 5	969	74
	NM_001169915.2	NP_001163386.1	4 - 5	969	74
	NM_139989.4	NP_648246.3	4 - 5	958	74
	NM_168283.4	NP_729395.2	4 - 5	969	74
	NM_168284.3	NP_729396.2	4 - 5	958	74
	NM_168286.4	NP_729398.1	3 - 4	456	74
	NM_176303.5	NP_788481.1	3 - 4	456	74
	NM_176304.5	NP_788482.1	3 - 4	456	74
	NM_176305.4	NP_788483.1	3 - 4	456	74
	NM_176306.3	NP_788484.2	2 - 3	596	74
GenBank mRNA	AY058415.1	-	4 - 5	936	74
	BT029127.1	-	4 - 5	938	74
	BT088849.1	-	3 - 4	432	74
	BT089012.1	-	3 - 4	451	74
	BT133470.1	-	4 - 5	967	74