Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Pgk
Entrez Gene ID: 33461
Gene Name: Phosphoglycerate kinase
Gene Aliases: 169C9S, CG3127, Dmel\CG3127, Dmel_CG3127, ESTS:169C9S, PGK, anon-sts18, l(2)23AB3, l(2)23Ac, pgk, pgk1
Chromosome Location: Chr.2L: 2746880 - 2753068 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0250906

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001273027.1	NP_001259956.1	2 - 3	549	51
	NM_001273028.1	NP_001259957.1	2 - 3	556	51
	NM_001273029.1	NP_001259958.1	2 - 3	554	51
	NM_001273030.1	NP_001259959.1	2 - 3	582	51
	NM_057328.4	NP_476676.1	1 - 2	175	51
GenBank mRNA	BT023910.1	-	-	185	51