Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: sima
Entrez Gene ID: 43580
Gene Name: similar
Gene Aliases: 7951, CG31031, CG45051, CG7951, DMU43090, Dmel\CG45051, Dmel_CG45051, HIF alpha, HIF-1, HIF-1alpha, HIF-alpha/sima, HIF1A, HIF1alpha, HIFalpha, Hif-1A, Hif1alpha, SIMA, Sima, bHLHe56, dHIF-1alpha, dHIF-alpha, l(3)j11B7
Chromosome Location: Chr.3R: 30058311 - 30121798 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0266411

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001276171.1	NP_001263100.1	3 - 4	1600	71
	NM_001300670.1	NP_001287599.1	9 - 10	2874	71
	NM_079845.4	NP_524584.2	9 - 10	3074	71