Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: spi
Entrez Gene ID: 35253
Gene Name: spitz
Gene Aliases: CG10334, CT29014, Dmel\CG10334, Dmel_CG10334, EP(2)2378, Spi, Spitz, anon-WO0118547.158, l(2)01068, l(2)37Fa, l(2)s3547, s-spi, sSpi
Chromosome Location: Chr.2L: 19567900 - 19577315 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0005672

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001032110.2	NP_001027281.1	3 - 3	1253	99
	NM_057561.6	NP_476909.2	2 - 2	1134	99
	NM_134291.5	NP_599118.2	3 - 3	1330	99
	NM_134292.3	NP_599119.2	2 - 2	1115	99
	NM_134293.3	NP_599120.2	3 - 3	1311	99
GenBank mRNA	BT044503.1	-	2 - 2	1144	99