Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Pfrx
Entrez Gene ID: 32938
Gene Name: 6-phosphofructo-2-kinase
Gene Aliases: CG3400, Dmel\CG3400, Dmel_CG3400, PFK2, Pfk2
Chromosome Location: Chr.X: 19495505 - 19509350 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0027621

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001258821.2	NP_001245750.1	3 - 3	1725	64
	NM_001258822.2	NP_001245751.1	2 - 2	1861	64
	NM_001258823.2	NP_001245752.1	2 - 2	2047	64
	NM_001298511.1	NP_001285440.1	2 - 2	1695	64
	NM_001298512.1	NP_001285441.1	2 - 2	2037	64
	NM_058103.3	NP_477451.1	2 - 2	2040	64
	NM_058104.5	NP_477452.3	2 - 2	2040	64
	NM_167651.2	NP_728249.1	2 - 2	1645	64
	NM_167652.2	NP_728250.1	2 - 2	1647	64
	NM_167653.3	NP_728251.1	2 - 2	1857	64
	NM_167654.2	NP_728252.1	2 - 2	1576	64
	NM_167655.2	NP_728253.1	2 - 2	1644	64
	NM_167656.2	NP_728254.1	2 - 2	1642	64
GenBank mRNA	AB023511.1	-	2 - 2	2040	64
GenBank mRNA	AY122115.1	-	2 - 2	2042	64