The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001278636.1 | NP_001265565.1 | 2 - 2 | 274 | 131 |
| NM_032565.4 | NP_115954.1 | 2 - 2 | 274 | 131 | |
| NR_103802.1 | 3 - 3 | 395 | 131 | ||
| GenBank mRNA | AF243433.1 | - | 2 - 2 | 263 | 131 |
| BC018478.1 | - | 2 - 2 | 240 | 131 | |
| BC021021.1 | - | 2 - 2 | 251 | 131 | |
| BC073152.1 | - | 3 - 3 | 395 | 131 | |
| BC092471.1 | - | 2 - 2 | 269 | 131 | |