Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: BRF1
Entrez Gene ID: 2972
Gene Name: BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit
Gene Aliases: BRF, BRF-1, CFDS, GTF3B, HEL-S-76p, TAF3B2, TAF3C, TAFIII90, TF3B90, TFIIIB90, hBRF
Chromosome Location: Chr.14: 105209286 - 105315577 on Build GRCh38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001242786.1	NP_001229715.1	17 - 18	2132	59
	NM_001242787.1	NP_001229716.1	17 - 18	2066	59
	NM_001242788.1	NP_001229717.1	16 - 17	2276	59
	NM_001242789.1	NP_001229718.1	12 - 13	1556	59
	NM_001519.3	NP_001510.2	17 - 18	2357	59
	NM_145685.2	NP_663718.1	13 - 14	2265	59
	XM_005267561.3	XP_005267618.1	17 - 18	2376	59
	XM_005267563.3	XP_005267620.1	13 - 14	3069	59
	XM_006720123.2	XP_006720186.1	12 - 13	2497	59
	XM_011536673.1	XP_011534975.1	16 - 17	2229	59
	XM_011536674.1	XP_011534976.1	13 - 14	2647	59
GenBank mRNA	AJ297407.1	-	13 - 14	2273	59
	AK096471.1	-	12 - 13	1556	59
	AK124665.1	-	7 - 8	1421	59
	AK125024.1	-	4 - 5	1871	59
	AK294899.1	-	17 - 18	2132	59
	AK295579.1	-	17 - 18	1979	59
	BC071637.1	-	12 - 13	1421	59
	BC086856.1	-	16 - 17	2009	59
	U28838.1	-	17 - 18	2354	59
	U75276.1	-	17 - 18	2061	59