Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: HUWE1
Entrez Gene ID: 10075
Gene Name: HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase
Gene Aliases: ARF-BP1, HECTH9, HSPC272, Ib772, LASU1, MULE, URE-B1, UREB1
Chromosome Location: Chr.X: 53532096 - 53686729 on Build GRCh38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_031407.6	NP_113584.3	82 - 83	13233	66
	XM_005261965.3	XP_005262022.1	81 - 82	13300	66
	XM_011530747.1	XP_011529049.1	83 - 84	13476	66
	XM_011530751.2	XP_011529053.1	82 - 83	13344	66
	XM_017029191.1	XP_016884680.1	81 - 82	16228	66
	XM_017029192.1	XP_016884681.1	81 - 82	16225	66
	XM_017029193.1	XP_016884682.1	81 - 82	16207	66
	XM_017029194.1	XP_016884683.1	81 - 82	16183	66
	XM_017029195.1	XP_016884684.1	81 - 82	16180	66
	XM_017029196.1	XP_016884685.1	81 - 82	16177	66
	XM_017029197.1	XP_016884686.1	80 - 81	16129	66
	XM_017029198.1	XP_016884687.1	82 - 83	13570	66
	XM_017029199.1	XP_016884688.1	83 - 84	13497	66
	XM_017029200.1	XP_016884689.1	82 - 83	13367	66
	XM_017029201.1	XP_016884690.1	82 - 83	13384	66
	XM_017029202.1	XP_016884691.1	82 - 83	13360	66
	XM_017029203.1	XP_016884692.1	82 - 83	13366	66
	XM_017029204.1	XP_016884693.1	80 - 81	15978	66
	XM_017029205.1	XP_016884694.1	79 - 80	15856	66
	XM_017029206.1	XP_016884695.1	78 - 79	15826	66
GenBank mRNA	AB002310.3	-	51 - 52	9284	66
	AB071605.1	-	55 - 56	9933	66
	AF057569.1	-	5 - 6	747	66
	AF161390.1	-	28 - 29	5480	66
	AK223167.1	-	7 - 8	1025	66
	AY772009.1	-	80 - 81	12878	66
	AY929612.1	-	79 - 80	12830	66
	BC002602.2	-	7 - 8	1034	66
	BC063505.1	-	6 - 7	873	66
	BC072421.1	-	11 - 12	1548	66
	BC107576.1	-	4 - 5	705	66
	CR456813.1	-	4 - 5	632	66
	DQ097177.1	-	82 - 83	13232	66