Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: PRNP
Entrez Gene ID: 5621
Gene Name: prion protein
Gene Aliases: ASCR, AltPrP, CD230, CJD, GSS, KURU, PRIP, PrP, PrP27-30, PrP33-35C, PrPc, p27-30
Chromosome Location: Chr.20: 4686151 - 4701588 on Build GRCh38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_000311.3	NP_000302.1	2 - 2	2538	116
	NM_001080121.1	NP_001073590.1	2 - 2	2533	116
	NM_001080122.1	NP_001073591.1	2 - 2	2529	116
	NM_001080123.1	NP_001073592.1	2 - 2	2402	116
	NM_001271561.1	NP_001258490.1	2 - 2	2538	116
	NM_183079.2	NP_898902.1	2 - 2	2534	116
GenBank mRNA	AK090575.1	-	3 - 3	2137	116
	AY008282.1	-	2 - 2	2209	116
	BC012844.1	-	2 - 2	2213	116
	BC016809.1	-	1 - 1	1366	116
	BC022532.1	-	2 - 2	2226	116
	M13899.1	-	2 - 2	2214	116