The assay primers and probes lie within a single exon. This assay will detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_021094.3 | NP_066580.1 | 14 - 14 | 2654 | 175 |
| NM_134431.3 | NP_602307.1 | 16 - 16 | 3124 | 175 | |
| XM_005253474.3 | XP_005253531.1 | 15 - 15 | 2545 | 175 | |
| XM_005253477.3 | XP_005253534.1 | 14 - 14 | 2432 | 175 | |
| XM_011520818.1 | XP_011519120.1 | 15 - 15 | 2552 | 175 | |
| XM_011520819.1 | XP_011519121.1 | 15 - 15 | 2699 | 175 | |
| XM_011520820.2 | XP_011519122.1 | 15 - 15 | 4021 | 175 | |
| XM_011520821.2 | XP_011519123.1 | 8 - 8 | 2824 | 175 | |
| GenBank mRNA | U21943.1 | - | 14 - 14 | 2456 | 175 |