The assay primers and probes lie within a single exon. This assay will detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001012727.1 | NP_001012745.1 | 5 - 5 | 1149 | 127 |
| NM_006412.3 | NP_006403.2 | 6 - 6 | 1245 | 127 | |
| GenBank mRNA | AF000237.1 | - | 6 - 6 | 1209 | 127 |
| AF011374.1 | - | 6 - 6 | 1206 | 127 | |
| BC000026.2 | - | 6 - 6 | 1188 | 127 | |
| BC004529.2 | - | 5 - 5 | 1083 | 127 | |
| BC007269.1 | - | 4 - 4 | 820 | 127 | |
| BC019292.1 | - | 6 - 6 | 1203 | 127 | |