The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_000141.4 | NP_000132.3 | 7 - 7 | 1540 | 146 |
| NM_001144915.1 | NP_001138387.1 | 6 - 6 | 945 | 146 | |
| NM_001144916.1 | NP_001138388.1 | 4 - 4 | 989 | 146 | |
| NM_001144918.1 | NP_001138390.1 | 5 - 5 | 1195 | 146 | |
| NM_001320654.1 | NP_001307583.1 | 6 - 6 | 924 | 146 | |
| NM_001320658.1 | NP_001307587.1 | 7 - 7 | 1540 | 146 | |
| NM_023029.2 | NP_075418.1 | 5 - 5 | 776 | 146 | |
| NR_073009.1 | 5 - 5 | 1195 | 294 | ||
| XM_017015921.1 | XP_016871410.1 | 7 - 7 | 1738 | 146 | |
| XM_017015923.1 | XP_016871412.1 | 5 - 5 | 776 | 146 | |
| XM_017015924.1 | XP_016871413.1 | 5 - 5 | 1393 | 146 | |
| XM_017015925.1 | XP_016871414.1 | 5 - 5 | 1393 | 146 | |
| GenBank mRNA | AK294026.1 | - | 7 - 7 | 945 | 146 |
| AK308621.1 | - | 4 - 4 | 989 | 146 | |
| BC037338.2 | - | 6 - 6 | 924 | 146 | |
| BC039243.1 | - | 5 - 5 | 1195 | 146 | |
| CX873387.1 | - | 3 - 3 | 303 | 146 | |
| EF057068.1 | - | 5 - 5 | 626 | 146 | |
| EU826598.1 | - | 6 - 6 | 893 | 146 | |
| EU826601.1 | - | 4 - 4 | 548 | 146 | |
| KP192325.1 | - | 2 - 2 | 338 | 146 | |
| KP192326.1 | - | 5 - 5 | 710 | 146 | |
| KP192327.1 | - | 5 - 5 | 710 | 146 | |
| KP192328.1 | - | 6 - 6 | 977 | 146 | |
| KP192329.1 | - | 4 - 4 | 632 | 146 | |
| KP192330.1 | - | 5 - 5 | 710 | 146 | |
| KP192331.1 | - | 6 - 6 | 977 | 146 | |
| M55614.1 | - | 6 - 6 | 905 | 155 | |
| M87770.1 | - | 7 - 7 | 1166 | 146 | |
| U11814.1 | - | 7 - 7 | 1151 | 294 | |
| X52832.1 | - | 7 - 7 | 1072 | 146 | |
| Z71929.1 | - | 6 - 6 | 905 | 146 | |