Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: PAPPA
Entrez Gene ID: 5069
Gene Name: pappalysin 1
Gene Aliases: ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A, PAPPA1
Chromosome Location: Chr.9: 116153752 - 116402321 on Build GRCh38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
GenBank mRNA	AF085834.1	-	1 - 1	351	75

Non Gene Transcripts

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	IMAGE Clone ID	Transcript Location	Amplicon Length
GenBankmRNA	BI056277.1	-	1			Chr. 9 - 116191939 - 116192357 [-] on Build GRCh38	73
GenBankmRNA	R69798.1	-				Chr. - - [-] on Build	75
GenBankmRNA	BQ024803.1	-	1			Chr. 9 - 116191955 - 116192520 [-] on Build GRCh38	75
GenBankmRNA	BQ370747.1	-	1	351		Chr. 9 - 116191949 - 116192354 [+] on Build GRCh38	75

Adjacent Gene Details

	Gene Name	Gene Symbol	Alias	Distance	Relative Direction	Location Chromosome
Upstream	long intergenic non-protein coding RNA 474	LINC00474	C9orf27, EST-YD1	-266.8kb	Anti-sense	Chr. - 115888265 - 115925098 on Build GRCh38
Downstream	PAPPA antisense RNA 1	PAPPA-AS1	DIPAS, NCRNA00156, PAPPA-AS, PAPPAAS, PAPPAS, TAF2C2, TAF4B, TAFII105	205.6kb	Anti-sense	Chr. - 116398157 - 116400606 on Build GRCh38

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