Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol
VEGFA
Entrez Gene ID
7422
Gene Name
vascular endothelial growth factor A
Gene Aliases
MVCD1, VEGF, VPF
Chromosome Location
Chr.6: 43770209 - 43786487 on Build GRCh38
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
RefSeq NM_001025366.2 NP_001020537.2 8 - 8 3214 134
NM_001025367.2 NP_001020538.2 8 - 8 3145 134
NM_001025368.2 NP_001020539.2 7 - 7 3091 134
NM_001025369.2 NP_001020540.2 7 - 7 3056 134
NM_001025370.2 NP_001020541.2 6 - 6 2959 134
NM_001033756.2 NP_001028928.1 7 - 7 3025 134
NM_001171622.1 NP_001165093.1 5 - 5 2929 134
NM_001171623.1 NP_001165094.1 8 - 8 3214 134
NM_001171624.1 NP_001165095.1 8 - 8 3163 134
NM_001171625.1 NP_001165096.1 8 - 8 3145 134
NM_001171626.1 NP_001165097.1 7 - 7 3091 134
NM_001171627.1 NP_001165098.1 7 - 7 3056 134
NM_001171628.1 NP_001165099.1 6 - 6 2959 134
NM_001171629.1 NP_001165100.1 7 - 7 3025 134
NM_001171630.1 NP_001165101.1 5 - 5 2929 134
NM_001204384.1 NP_001191313.1 7 - 7 3031 134
NM_001204385.1 NP_001191314.1 7 - 7 3031 134
NM_001287044.1 NP_001273973.1 7 - 7 2104 134
NM_001317010.1 NP_001303939.1 7 - 7 3091 134
NM_003376.5 NP_003367.4 8 - 8 3163 134
GenBank mRNA AB209485.1 - 3 - 3 11725 134
AF024710.1 - 1 - 1 1477 134
AK125666.1 - 1 - 1 2009 134
BC065522.1 - 6 - 6 2386 134

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