The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001308373.1 | NP_001295302.1 | 5 - 5 | 785 | 243 |
| NM_012181.4 | NP_036313.3 | 5 - 5 | 788 | 246 | |
| XM_011527863.1 | XP_011526165.1 | 5 - 5 | 846 | 246 | |
| XM_011527864.1 | XP_011526166.1 | 4 - 4 | 677 | 246 | |
| GenBank mRNA | AK026952.1 | - | - | 1376 | 246 |
| AK300571.1 | - | 5 - 5 | 759 | 243 | |
| AK313363.1 | - | 5 - 5 | 719 | 246 | |
| AY225339.1 | - | 4 - 4 | 649 | 243 | |
| AY278607.1 | - | 5 - 5 | 712 | 243 | |
| BC009966.1 | - | 4 - 4 | 492 | 243 | |
| BX538124.1 | - | 5 - 5 | 766 | 246 | |
| BX647405.1 | - | 5 - 5 | 765 | 246 | |
| BX647720.1 | - | 5 - 5 | 723 | 246 | |