The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_000022.3 | NP_000013.2 | 5 - 5 | 620 | 115 |
| NM_001322051.1 | NP_001308980.1 | 5 - 5 | 620 | 115 | |
| NR_136160.1 | 5 - 5 | 620 | 115 | ||
| GenBank mRNA | AK223397.1 | - | 5 - 5 | 570 | 115 |
| AL832305.1 | - | 2 - 2 | 143 | 115 | |
| BC007678.2 | - | 5 - 5 | 581 | 115 | |
| BC040226.1 | - | 5 - 5 | 587 | 115 | |
| CD014056.1 | - | 5 - 5 | 553 | 115 | |
| KJ534760.1 | - | 5 - 5 | 469 | 115 | |
| KU177892.1 | - | 5 - 5 | 469 | 115 | |
| KU177893.1 | - | 5 - 5 | 469 | 115 | |
| X02994.1 | - | 5 - 5 | 564 | 115 | |