The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001145873.1 | NP_001139345.1 | 8 - 8 | 212 | |
| NM_001768.6 | NP_001759.3 | 5 - 5 | 212 | ||
| NM_171827.3 | NP_741969.1 | 4 - 4 | 1415 | 101 | |
| NR_027353.1 | 6 - 6 | 212 | |||
| GenBank mRNA | AK097942.1 | - | 6 - 6 | 212 | |
| AK124156.1 | - | 8 - 8 | 212 | ||
| AK300089.1 | - | 6 - 6 | 212 | ||
| AY039664.1 | - | 5 - 5 | 212 | ||
| BC025715.1 | - | 5 - 5 | 212 | ||
| M12824.1 | - | 5 - 5 | 212 | ||
| M12828.1 | - | 5 - 5 | 212 | ||