The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_002187.2 | NP_002178.2 | 3 - 3 | 374 | 120 |
GenBank mRNA | AF180563.1 | - | 2 - 2 | 338 | 120 |
BC067498.1 | - | 2 - 2 | 336 | 120 | |
BC067499.1 | - | 2 - 2 | 336 | 120 | |
BC067500.1 | - | 2 - 2 | 336 | 120 | |
BC067501.1 | - | 2 - 2 | 336 | 120 | |
BC067502.1 | - | 2 - 2 | 336 | 120 | |
BC074723.2 | - | 3 - 3 | 363 | 120 | |
M65272.1 | - | 3 - 3 | 374 | 120 | |
M65290.1 | - | 2 - 2 | 345 | 120 |