Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Sema3b
Entrez Gene ID: 20347
Gene Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Gene Aliases: SemA, Semaa, sema5, semaV
Chromosome Location: Chr.9: 107597674 - 107609303 on Build GRCm38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001042779.2	NP_001036244.1	12 - 13	1395	75
	NM_001291537.1	NP_001278466.1	12 - 13	1659	75
	NM_001291538.1	NP_001278467.1	12 - 13	1653	75
	NM_001291539.1	NP_001278468.1	11 - 12	1336	75
	NM_009153.3	NP_033179.2	10 - 11	1205	75
	NR_111986.1		10 - 11	1215	75
GenBank mRNA	AK014333.1	-	10 - 11	1151	75
	AK033597.1	-	11 - 12	1346	75
	AK037034.1	-	12 - 13	1384	75
	AK043634.1	-	11 - 12	1289	75
	AK048364.1	-	11 - 12	1617	75
	BC090669.1	-	11 - 12	1316	75
	BC150758.1	-	9 - 10	1051	75
	CN538654.1	-	3 - 4	251	75
	X85990.1	-	10 - 11	1202	75