The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_019745.4 | NP_062719.2 | 3 - 3 | 392 | 128 |
| GenBank mRNA | AF159368.1 | - | 3 - 3 | 295 | 128 |
| AK005594.1 | - | 3 - 3 | 321 | 128 | |
| AK075926.1 | - | 3 - 3 | 340 | 128 | |
| AK077308.1 | - | 3 - 3 | 369 | 128 | |
| AK137198.1 | - | 3 - 3 | 362 | 128 | |
| AK149182.1 | - | 3 - 3 | 298 | 128 | |
| AK160234.1 | - | 3 - 3 | 342 | 128 | |
| AK168857.1 | - | 3 - 3 | 326 | 128 | |
| AK169689.1 | - | 3 - 3 | 326 | 128 | |
| BC019650.1 | - | 3 - 3 | 332 | 128 | |
| BC086778.1 | - | 3 - 3 | 311 | 128 | |