The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_024414.2 | NP_077725.1 | 3 - 3 | 420 | 153 |
GenBank mRNA | BC132042.1 | - | 3 - 3 | 215 | 153 |
BC132068.1 | - | 3 - 3 | 215 | 153 | |
D29743.1 | - | 3 - 3 | 247 | 153 |