Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Sema4b
Entrez Gene ID: 20352
Gene Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
Gene Aliases: SemC, Semac, mKIAA1745
Chromosome Location: Chr.7: 80186841 - 80226527 on Build GRCm38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_013659.4	NP_038687.2	3 - 4	573	84
GenBank mRNA	AK028896.1	-	3 - 4	536	84
	AK030331.1	-	2 - 3	573	84
	AK134973.1	-	2 - 3	501	84
	AK145570.1	-	3 - 4	574	84
	AK154969.1	-	2 - 3	354	84
	AK156620.1	-	2 - 3	407	84
	AK173249.1	-	3 - 4	521	84
	DQ073331.1	-	2 - 3	329	84