The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_130889.2 | NP_570959.1 | 4 - 4 | 707 | 191 |
| GenBank mRNA | AB025582.1 | - | 4 - 4 | 528 | 191 |
| AK136648.1 | - | 4 - 4 | 541 | 191 | |
| AK165807.1 | - | 4 - 4 | 777 | 191 | |
| BC003489.1 | - | 4 - 4 | 725 | 191 | |
| BC005628.1 | - | 4 - 4 | 733 | 191 | |