The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_145549.1 | NP_663524.1 | - | 536 | 106 |
| GenBank mRNA | AK142188.1 | - | 3 - 3 | 741 | 106 |
| AK143777.1 | - | - | 594 | 106 | |
| AK220422.1 | - | - | 485 | 106 | |
| BC020077.1 | - | - | 536 | 106 | |
| BC112327.1 | - | - | 387 | 106 | |