The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001130408.1 | NP_001123880.1 | 4 - 4 | 694 | 165 |
| NM_007476.3 | NP_031502.1 | 4 - 4 | 502 | 165 | |
| GenBank mRNA | AK148458.1 | - | 4 - 4 | 466 | 165 |
| AK148873.1 | - | 4 - 4 | 456 | 165 | |
| AK150092.1 | - | 4 - 4 | 475 | 165 | |
| AK150699.1 | - | 4 - 4 | 452 | 165 | |
| AK151223.1 | - | 4 - 4 | 452 | 165 | |
| AK151225.1 | - | 4 - 4 | 475 | 165 | |
| AK151696.1 | - | 4 - 4 | 428 | 165 | |
| AK151821.1 | - | 4 - 4 | 450 | 165 | |
| AK152015.1 | - | 4 - 4 | 448 | 165 | |
| AK152382.1 | - | 4 - 4 | 450 | 165 | |
| AK152479.1 | - | 4 - 4 | 709 | 165 | |
| AK153132.1 | - | 4 - 4 | 469 | 165 | |
| AK159174.1 | - | 4 - 4 | 452 | 165 | |
| AK167000.1 | - | 4 - 4 | 431 | 165 | |
| AK167129.1 | - | 4 - 4 | 465 | 165 | |
| AK167543.1 | - | 4 - 4 | 452 | 165 | |
| AK167931.1 | - | 4 - 4 | 466 | 165 | |
| AK168081.1 | - | 4 - 4 | 639 | 165 | |
| AK168686.1 | - | 4 - 4 | 452 | 165 | |
| AK169166.1 | - | 4 - 4 | 450 | 165 | |
| AK170092.1 | - | 4 - 4 | 454 | 165 | |
| AK170586.1 | - | 4 - 4 | 427 | 165 | |
| BC021403.1 | - | 3 - 3 | 445 | 165 | |
| BC031986.1 | - | 3 - 3 | 419 | 165 | |
| D87898.1 | - | 4 - 4 | 449 | 165 | |