The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_011295.6 | NP_035425.2 | 3 - 3 | 167 | 149 |
| GenBank mRNA | AK002848.1 | - | 3 - 3 | 159 | 149 |
| AK008057.1 | - | 3 - 3 | 159 | 149 | |
| AK032757.1 | - | 3 - 3 | 159 | 149 | |
| AK166923.1 | - | 3 - 3 | 157 | 149 | |
| AK166992.1 | - | 3 - 3 | 162 | 149 | |
| AK168324.1 | - | 3 - 3 | 159 | 149 | |
| AK169215.1 | - | 3 - 3 | 159 | 149 | |
| BC002079.1 | - | 3 - 3 | 143 | 149 | |
| BC089338.1 | - | 3 - 3 | 155 | 149 | |
| BC089339.1 | - | 3 - 3 | 141 | 149 | |
| BC090257.1 | - | 3 - 3 | 134 | 149 | |
| BC092044.1 | - | 3 - 3 | 141 | 149 | |
| BC096653.1 | - | 3 - 3 | 170 | 149 | |
| BC099377.1 | - | 3 - 3 | 143 | 149 | |
| BY128264.1 | - | 3 - 3 | 169 | 149 | |
| X15962.1 | - | 2 - 2 | 132 | 149 | |