Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol
Hist1h2af
Entrez Gene ID
319173
Gene Name
histone cluster 1, H2af
Gene Aliases
H2a-221
Chromosome Location
Chr.13: 23533911 - 23534378 on Build GRCm38
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
RefSeq NM_175661.2 NP_783592.1 1 - 1 55 129
GenBank mRNA BC125011.1 - 1 - 1 55 129

Gene Symbol
Hist1h2ad
Entrez Gene ID
319165
Gene Name
histone cluster 1, H2ad
Gene Aliases
-
Chromosome Location
Chr.13: 23574381 - 23574915 on Build GRCm38
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
RefSeq NM_178188.4 NP_835495.1 1 - 1 145 129
GenBank mRNA BC119295.1 - 1 - 1 145 129
BC119297.1 - 1 - 1 145 129

Gene Symbol
Hist1h2ag
Entrez Gene ID
319167
Gene Name
histone cluster 1, H2ag
Gene Aliases
-
Chromosome Location
Chr.13: 22042478 - 22042949 on Build GRCm38
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
RefSeq NM_178186.3 NP_835493.1 1 - 1 77 129
GenBank mRNA BC065803.1 - 1 - 1 77 129

Gene Symbol
Hist1h2an
Entrez Gene ID
319170
Gene Name
histone cluster 1, H2an
Gene Aliases
-
Chromosome Location
Chr.13: 21786772 - 21787218 on Build GRCm38
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
RefSeq NM_178184.2 NP_835491.1 1 - 1 55 129

Gene Symbol
Hist1h2ap
Entrez Gene ID
319171
Gene Name
histone cluster 1, H2ap
Gene Aliases
ENSMUSG00000060262, Gm11276, OTTMUSG00000000448
Chromosome Location
Chr.13: 21833024 - 21833475 on Build GRCm38
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
GenBank mRNA BC110626.1 - 1 - 1 55 129
BC133661.1 - 1 - 1 55 129

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